Neural stem cells : methods and protocols / edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos.
Record details
- ISBN: 0896039641 (alk. paper)
- Physical Description: xiv, 381 p. : il. ; 24 cm.
- Publisher: Totowa, NJ : Humana Press, c2002.
Content descriptions
| Bibliography, etc. Note: | Incluye bibliografía e índice. |
| Language Note: | English |
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| Subject: | Neuronas > Manuales de laboratorio. Células madre > Manuales de laboratorio. |
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- 1 of 1 copy available at IPICYT.
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| Location | Call Number / Copy Notes | Barcode | Shelving Location | Status | Due Date |
|---|---|---|---|---|---|
| Biblioteca Ipicyt | QP357 N4.Z5 2002 | BIO00134 | Coleccion General | Available | - |
| Machine generated contents note: Prefacev | ||
| Contributors xi | ||
| PART I ISOLATION AND CULTURE OF NSCs | ||
| 1 Neural Differentiation of Embryonic Stem Cells | ||
| K. Sue O'Shea | ||
| 2 Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens | ||
| Eric D. Laywell, Valery G. Kukekov, Oleg Suslov, | ||
| Tong Zheng, and Dennis A. Steindler 15 | ||
| 3 Isolation of Stem and Precursor Cells from Fetal Tissue | ||
| Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao29 | ||
| 4 Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb | ||
| Susan C. Barnett and A. Jane Roskams 41 | ||
| 5 Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium | ||
| Edmund Au and A. Jane Roskams49 | ||
| 6 Production of Immortalized Human Neural Crest Stem Cells | ||
| Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai, | ||
| Myung A. Lee, and Jung H. Bang55 | ||
| 7 Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization | ||
| Lamya S. Shihabuddin67 | ||
| 8 Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood | ||
| Shijie Song and J. Sanchez-Ramos79 | ||
| 9 Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers | ||
| Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer 89 | ||
| PART II CHARACTERIZATION OF NSCs IN VITRO | ||
| A. CELLULAR TECHNIQUES | ||
| 10 Analysis of Cell Generation in the Telencephalic Neuroepithelium | ||
| Takao Takahashi, Verne S. Caviness, Jr., | ||
| and Pradeep G. Bhide101 | ||
| 11 Clonal Analyses and Cryopreservation | ||
| of Neural Stem Cell Cultures | ||
| Angelo L. Vescovi, Rossella Galli, and Angela Gritti115 | ||
| 12 Assessing the Involvement of Telomerase in Stem Cell Biology | ||
| Mark P. Mattson, Peisu Zhang, and Weiming Fu 125 | ||
| 13 Detection of Telomerase Activity in Neural Cells | ||
| Karen R. Prowse137 | ||
| 14 In Vitro Assays for Neural Stem Cell Differentiation | ||
| Marcel M. Daadi 149 | ||
| 15 Electron Microscopy and Lac-Z Labeling | ||
| Bela Kosaras and Evan Snyder157 | ||
| B. ELECTROPHYSIOLOGICAL TECHNIQUES | ||
| 16 Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells | ||
| David S. K. Magnuson and Dante J. Morassutti179 | ||
| C. MOLECULAR TECHNIQUES | ||
| 17 Fluorescence In Situ Hybridization | ||
| Barbara A. Tate and Rachel L. Ostroff 189 | ||
| 18 RT-PCR Analyses of Differential Gene Expression | ||
| in ES-Derived Neural Stem Cells | ||
| Theresa E. Gratsch197 | ||
| 19 Differential Display: Isolation of Novel Genes | ||
| Theresa E. Gratsch213 | ||
| 20 Cell Labeling and Gene Misexpression by Electroporation | ||
| Terence J. Van Raay and Michael R. Stark223 | ||
| 21 Gene Therapy Using Neural Stem Cells | ||
| Luciano Conti and Elena Cattaneo 233 | ||
| 22 Modeling Brain Pathologies Using Neural Stem Cells | ||
| Simonetta Siplone and Elena Cattaneo245 | ||
| PART III UTILIZATION/CHARACTERIZATION OF NSCs IN VivO | ||
| A. ENDOGENOUS POOLS OF STEM/PROGENITOR CELLS | ||
| 23 Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model | ||
| Marcel M. Daadi 265 | ||
| 24 Identification of Musashil-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods | ||
| Yonehiro Kanemura, Shin-ichi Sakakibara, | ||
| and Hideyuki Okano273 | ||
| 25 Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo | ||
| Sanjay S. P. Magavi and Jeffrey D. Macklis283 | ||
| 26 Immunocytochemical Analysis of Neuronal Differentiation | ||
| Sanjay S. P. Magavi and Jeffrey D. Macklis291 | ||
| 27 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold | ||
| Lisa A. Catapano, Sanjay S. P. Magavi, | ||
| and Jeffrey D. Macklis299 | ||
| B. TRANSPLANTATION | ||
| 28 Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS | ||
| Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore307 | ||
| 29 Optimizing Stem Cell Grafting into the CNS | ||
| Scott R. Whittemore, Y. Ping Zhang, Christopher B. Shields, | ||
| Dante J. Morassutti, and David S. K. Magnuson319 | ||
| 30 Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos | ||
| Lorenzo M agrassi327 | ||
| 31 Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells | ||
| Tanja Zigova and Mary B. Newman341 | ||
| 32 Routes of Stem Cell Administration in the Adult Rodent | ||
| Alison E. Willing, Svitlana Garbuzova-Davis, | ||
| Paul R. Sanberg, and Samuel Saporta357 | ||
| Index3 7 5. |
